Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP1816a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1816a, RRID:AB_2059363
- Product name
- ATG12 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Interaction of caveolin-1 with ATG12-ATG5 system suppresses autophagy in lung epithelial cells.
Activation of autophagy in mesenchymal stem cells provides tumor stromal support.
PUMA- and Bax-induced autophagy contributes to apoptosis.
Absence of the type I IFN system in EC cells: transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated.
Chen ZH, Cao JF, Zhou JS, Liu H, Che LQ, Mizumura K, Li W, Choi AM, Shen HH
American journal of physiology. Lung cellular and molecular physiology 2014 Jun 1;306(11):L1016-25
American journal of physiology. Lung cellular and molecular physiology 2014 Jun 1;306(11):L1016-25
Activation of autophagy in mesenchymal stem cells provides tumor stromal support.
Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR
Carcinogenesis 2011 Jul;32(7):964-72
Carcinogenesis 2011 Jul;32(7):964-72
PUMA- and Bax-induced autophagy contributes to apoptosis.
Yee KS, Wilkinson S, James J, Ryan KM, Vousden KH
Cell death and differentiation 2009 Aug;16(8):1135-45
Cell death and differentiation 2009 Aug;16(8):1135-45
Absence of the type I IFN system in EC cells: transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated.
Harada H, Willison K, Sakakibara J, Miyamoto M, Fujita T, Taniguchi T
Cell 1990 Oct 19;63(2):303-12
Cell 1990 Oct 19;63(2):303-12
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-APG12L Pab (Cat. #AP1816a) in Hela cell lysate. APG12L (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. "
- Primary Ab dilution
- 1:200
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody (N-term) (Cat.#AP1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100