Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 36-6400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins.
Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12–5-16L2 complex.
Small molecules enhance autophagy and reduce toxicity in Huntington's disease models.
Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and alpha-synuclein.
Al-Zeer MA, Al-Younes HM, Lauster D, Abu Lubad M, Meyer TF
Autophagy 2013 Jan;9(1):50-62
Autophagy 2013 Jan;9(1):50-62
Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12–5-16L2 complex.
Ishibashi K, Fujita N, Kanno E, Omori H, Yoshimori T, Itoh T, Fukuda M
Autophagy 2011 Dec;7(12):1500-13
Autophagy 2011 Dec;7(12):1500-13
Small molecules enhance autophagy and reduce toxicity in Huntington's disease models.
Sarkar S, Perlstein EO, Imarisio S, Pineau S, Cordenier A, Maglathlin RL, Webster JA, Lewis TA, O'Kane CJ, Schreiber SL, Rubinsztein DC
Nature chemical biology 2007 Jun;3(6):331-8
Nature chemical biology 2007 Jun;3(6):331-8
Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and alpha-synuclein.
Sarkar S, Davies JE, Huang Z, Tunnacliffe A, Rubinsztein DC
The Journal of biological chemistry 2007 Feb 23;282(8):5641-52
The Journal of biological chemistry 2007 Feb 23;282(8):5641-52
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Apg12-transfected 293 cells using Rb anti-Apg12 (Product # 36-6400).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ATG12 Polyclonal Antibody (Product # 36-6400) and a 60kDa band corresponding to ATG12 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of HCT 116 (Lane 1), HCT 116 treated with rapamycin (50uM, 16 Hrs) (Lane 2), U-2 OS (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). ATG12 gets conjugated with ATG5. Expression of ATG12/5 is upregulated upon treatment with rapamycin in HCT116 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
