- GTX124181 - GeneTex ATG12 antibody

Submitted references Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.

Hsu HL, Liao PL, Cheng YW, Huang SH, Wu CH, Li CH, Kang JJ
International journal of molecular sciences 2019 Jan 3;20(1)

Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.

Liu YH, Weng YP, Tsai HY, Chen CJ, Lee DY, Hsieh CL, Wu YC, Lin JY
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192

miR-30a Remodels Subcutaneous Adipose Tissue Inflammation to Improve Insulin Sensitivity in Obesity.

Koh EH, Chernis N, Saha PK, Xiao L, Bader DA, Zhu B, Rajapakshe K, Hamilton MP, Liu X, Perera D, Chen X, York B, Trauner M, Coarfa C, Bajaj M, Moore DD, Deng T, McGuire SE, Hartig SM
Diabetes 2018 Dec;67(12):2541-2553

Helicobacter pylori cholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages.

Lai CH, Huang JC, Cheng HH, Wu MC, Huang MZ, Hsu HY, Chen YA, Hsu CY, Pan YJ, Chu YT, Chen TJ, Wu YF, Sit WY, Liu JS, Chiu YF, Wang HJ, Wang WC
Cellular microbiology 2018 Dec;20(12):e12947

Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.

Kuo SM, Chen CJ, Chang SC, Liu TJ, Chen YH, Huang SY, Shih SR
mBio 2017 Jun 13;8(3)

Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.

Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437

The inhibition of lung cancer cell migration by AhR-regulated autophagy.

Tsai CH, Li CH, Cheng YW, Lee CC, Liao PL, Lin CH, Huang SH, Kang JJ
Scientific reports 2017 Feb 14;7:41927

Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells.

Yen JH, Wu PS, Chen SF, Wu MJ
International journal of molecular sciences 2017 Apr 17;18(4)

A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.

Wei RJ, Lin SS, Wu WR, Chen LR, Li CF, Chen HD, Chou CT, Chen YC, Liang SS, Chien ST, Shiue YL
Toxicology and applied pharmacology 2016 Nov 15;311:88-98

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 42263

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE GTX124181 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: NIH-3T3 12% SDS PAGE GTX124181 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: Rat brain 12% SDS PAGE GTX124181 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG12 antibody detects ATG12 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of ATG12 protein from 293T whole cell extracts using 5 ?g of ATG12 antibody (GTX124181).Western blot analysis was performed using ATG12 antibody (GTX124181).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Validation comment: IP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 £gg of ATG12 antibody (GTX124181).Western blot analysis was performed using ATG12 antibody (GTX124181).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ATG12 antibody (GTX124181) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ATG12 antibody (GTX124181) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG12 antibody detects ATG12 protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ATG12 antibody (GTX124181) diluted at 1:500.

GTX124181

Antibody data