Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-48069 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CPLX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects endogenous human, mouse and rat Complexin-2 in Western blots.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-human/mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48069) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Complexin-2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CPLX2 in mouse, human, and rat brain tissue. Samples were incubated in CPLX2 polyclonal antibody (Product # PA5-48069) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Complexin‚2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CPLX2 in SH‚SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid prior to immersion fixation. Samples were incubated in CPLX2 polyclonal antibody (Product # PA5-48069) followed by NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the nuclei were counterstained with DAPI (blue). Complexin-2 immunoreactivity was localized to synaptic vesicles.