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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [4]
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- Product number
- PA5-58169 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH3PXD2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PDPSGKELDT VPAKGRQNEG KSDSLEKIER RVQALNTVNQ SKKATPPIPS KPPGGFGKTS GTPAVKMRNG VRQVAVRPQS VFVSP
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SH3PXD2A in human cell line HeLa using a SH3PXD2A Polyclonal Antibody (Product # PA5-58169).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SH3PXD2A in mouse cell line NIH-3T3 and rat cell line NBT-II using a SH3PXD2A Polyclonal Antibody (Product # PA5-58169).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SH3PXD2A in mouse cell line NIH-3T3 and rat cell line NBT-II using a SH3PXD2A Polyclonal Antibody (Product # PA5-58169).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SH3PXD2A Polyclonal Antibody (Product # PA5-58169) and ~130kDa band corresponding to SH3PXD2A was observed in HeLa and A-375 cells and not in SK-BR-3 which is reported to be negative. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SH3PXD2A in human placenta using a SH3PXD2A Polyclonal Antibody (Product # PA5-58169) shows strong membranous positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SH3PXD2A in human prostate using SH3PXD2A Polyclonal Antibody (Product # PA5-58169) shows moderate cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SH3PXD2A in human skin using SH3PXD2A Polyclonal Antibody (Product # PA5-58169) shows moderate cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SH3PXD2A in human pancreas using SH3PXD2A Polyclonal Antibody (Product # PA5-58169) shows very weak positivity in exocrine glandular cells and strong positivity in endocrine glandular cells.
