Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-32971 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX3 Monoclonal Antibody (6G8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6G8
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX3 in HeLa, 3T3, C6, COS7, K562 and Jurkat cell lysate. Sample was incubated with DDX3 monoclonal antibody (Product # MA5-32971) using a 1:1000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ATP-dependent RNA helicase DDX3X was achieved by transfecting A-375 with DDX3X specific siRNAs (Silencer® select Product # s4005, s4006). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATP-dependent RNA helicase DDX3X knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with DDX3 Monoclonal Antibody (6G8) (Product # MA5-32971, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP-dependent RNA helicase DDX3X.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using DDX3 Monoclonal Antibody (6G8) (Product # MA5-32971) and a ~75 kDa band corresponding to DDX3X was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), A-375 (Lane 4), HCT 116 (Lane 5), C2C12 (Lane 6) and A-431 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DDX3 in HeLa cells. Sample was incubated with monoclonal DDX3 antibody (Product # MA5-32971) using a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of DDX3 in HeLa cells. Sample was incubated with DDX3 monoclonal antibody (Product # MA5-32971).