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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-61107 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SRP9 Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
The epitope is mapped to a region b
etween residue 36 to 86 of human Si
gnal recognition particle 9 kDa pro
tein using the numbering given in e
ntry P49458.2 (GeneID 6726).- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SRP9 Antibody [NBP2-61107] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using lysis buffer at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SRP9 Antibody [NBP2-61107] - Detection of human SRP9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRP9 antibody NBP2-61107 used for IP at 6 ug per reaction. SRP9 was also immunoprecipitated by another rabbit anti-SRP9 antibody. For blotting immunoprecipitated SRP9, NBP2-61107 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
