HPA023885

antibody from Atlas Antibodies

Targeting: DMD BMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA023885

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023885, RRID:AB_1847931

Product name

Anti-DMD

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQP
LEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVN
TEWEKLNLHSADWQRKIDETLERLQELQEATDELD
LKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALR
GEIAPLKENV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skeletal muscle and tonsil tissues using HPA023885 antibody. Corresponding DMD RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows strong membranous positivity in myocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows no positivity in lymphoid cells as expected.

Sample type

HUMAN