PA5-63534

antibody from Invitrogen Antibodies

Targeting: DNAH12 DHC3, DLP12, DNAH12L, DNAH7L, Dnahc3, DNHD2, FLJ40427, FLJ44290, hdhc3, HL-19

Antibody data

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Validation data

Product number: PA5-63534 - Provider product page
Provider: Invitrogen Antibodies
Product name: DNAH12 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: LLCANLLLAR KEIEYQELMF LLTGGVSLKS AEKNPDPTWL QDKSWEEICR ASEFPAFRGL RQHFCEHIYE WREIYDSKEP HNAKFPAPMD KNLNELQKII
Concentration: 0.4 mg/mL

DNAH12 protein structure - PA5-63534 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of DNAH12 was performed on human pancreas tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in 3% BSA-PBS for 30 min at room temperature. Tissues were probed with a DNAH12 polyclonal antibody (Product # PA5-63534) diluted 1:500 in 3% BSA-PBS overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were takenat 20X magnification. Data courtesy of Antibody Data Exchange Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical staining of DNAH12 in human liver using DNAH12 Polyclonal Antibody (Product # PA5-63534).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical staining of DNAH12 in human fallopian tube shows moderate positivity in ciliated cells. Samples were probed using a DNAH12 Polyclonal Antibody (Product # PA5-63534).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of DNAH12 in human cerebral cortex using DNAH12 Polyclonal Antibody (Product # PA5-63534).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of DNAH12 in human fallopian tube and prostate tissues using DNAH12 Polyclonal Antibody (Product # PA5-63534). Corresponding DNAH12 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of DNAH12 in human cerebral cortex, fallopian tube, liver and prostate using DNAH12 Polyclonal Antibody (Product # PA5-63534) (A) shows similar protein distribution across tissues to an independent DNAH12 Polyclonal Antibody (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of DNAH12 in human prostate using DNAH12 Polyclonal Antibody (Product # PA5-63534) shows low expression as expected.