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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Western blot [6]
- Immunohistochemistry [7]
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- Product number
- NBP1-83220 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83220, RRID:AB_11023683
- Product name
- Rabbit Polyclonal PGRMC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PGRMC1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance.
Triple-responsive expansile nanogel for tumor and mitochondria targeted photosensitizer delivery.
Progesterone receptor membrane component 1 as the mediator of the inhibitory effect of progestins on cytokine-induced matrix metalloproteinase 9 activity in vitro.
Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells.
Progesterone antagonism of neurite outgrowth depends on microglial activation via Pgrmc1/S2R.
Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cells.
Differential responses of progesterone receptor membrane component-1 (Pgrmc1) and the classical progesterone receptor (Pgr) to 17β-estradiol and progesterone in hippocampal subregions that support synaptic remodeling and neurogenesis.
Evidence for a genomic mechanism of action for progesterone receptor membrane component-1.
Progesterone regulation of progesterone receptor membrane component 1 (PGRMC1) sumoylation and transcriptional activity in spontaneously immortalized granulosa cells.
Expression of progesterone receptor membrane component-1 in bovine reproductive system during estrous cycle.
Progesterone inhibits apoptosis in part by PGRMC1-regulated gene expression.
Progesterone stimulates the proliferation of female and male cholangiocytes via autocrine/paracrine mechanisms.
Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, Sugase K, Shimamura T, Ohmura M, Muraoka K, Yamamoto A, Uchida T, Iwata S, Yamaguchi Y, Krayukhina E, Noda M, Handa H, Ishimori K, Uchiyama S, Kobayashi T, Suematsu M
Nature communications 2016 Mar 18;7:11030
Nature communications 2016 Mar 18;7:11030
Triple-responsive expansile nanogel for tumor and mitochondria targeted photosensitizer delivery.
He H, Cattran AW, Nguyen T, Nieminen AL, Xu P
Biomaterials 2014 Nov;35(35):9546-53
Biomaterials 2014 Nov;35(35):9546-53
Progesterone receptor membrane component 1 as the mediator of the inhibitory effect of progestins on cytokine-induced matrix metalloproteinase 9 activity in vitro.
Allen TK, Feng L, Grotegut CA, Murtha AP
Reproductive sciences (Thousand Oaks, Calif.) 2014 Feb;21(2):260-8
Reproductive sciences (Thousand Oaks, Calif.) 2014 Feb;21(2):260-8
Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells.
Hulce JJ, Cognetta AB, Niphakis MJ, Tully SE, Cravatt BF
Nature methods 2013 Mar;10(3):259-64
Nature methods 2013 Mar;10(3):259-64
Progesterone antagonism of neurite outgrowth depends on microglial activation via Pgrmc1/S2R.
Bali N, Arimoto JM, Morgan TE, Finch CE
Endocrinology 2013 Jul;154(7):2468-80
Endocrinology 2013 Jul;154(7):2468-80
Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cells.
Peluso JJ, Yuan A, Liu X, Lodde V
Biology of reproduction 2013 Jan;88(1):20
Biology of reproduction 2013 Jan;88(1):20
Differential responses of progesterone receptor membrane component-1 (Pgrmc1) and the classical progesterone receptor (Pgr) to 17β-estradiol and progesterone in hippocampal subregions that support synaptic remodeling and neurogenesis.
Bali N, Arimoto JM, Iwata N, Lin SW, Zhao L, Brinton RD, Morgan TE, Finch CE
Endocrinology 2012 Feb;153(2):759-69
Endocrinology 2012 Feb;153(2):759-69
Evidence for a genomic mechanism of action for progesterone receptor membrane component-1.
Peluso JJ, DeCerbo J, Lodde V
Steroids 2012 Aug;77(10):1007-12
Steroids 2012 Aug;77(10):1007-12
Progesterone regulation of progesterone receptor membrane component 1 (PGRMC1) sumoylation and transcriptional activity in spontaneously immortalized granulosa cells.
Peluso JJ, Lodde V, Liu X
Endocrinology 2012 Aug;153(8):3929-39
Endocrinology 2012 Aug;153(8):3929-39
Expression of progesterone receptor membrane component-1 in bovine reproductive system during estrous cycle.
Luciano AM, Corbani D, Lodde V, Tessaro I, Franciosi F, Peluso JJ, Modina S
European journal of histochemistry : EJH 2011;55(3):e27
European journal of histochemistry : EJH 2011;55(3):e27
Progesterone inhibits apoptosis in part by PGRMC1-regulated gene expression.
Peluso JJ, Liu X, Gawkowska A, Lodde V, Wu CA
Molecular and cellular endocrinology 2010 May 14;320(1-2):153-61
Molecular and cellular endocrinology 2010 May 14;320(1-2):153-61
Progesterone stimulates the proliferation of female and male cholangiocytes via autocrine/paracrine mechanisms.
Glaser S, DeMorrow S, Francis H, Ueno Y, Gaudio E, Vaculin S, Venter J, Franchitto A, Onori P, Vaculin B, Marzioni M, Wise C, Pilanthananond M, Savage J, Pierce L, Mancinelli R, Alpini G
American journal of physiology. Gastrointestinal and liver physiology 2008 Jul;295(1):G124-G136
American journal of physiology. Gastrointestinal and liver physiology 2008 Jul;295(1):G124-G136
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PGRMC1 Antibody [NBP1-83220] - Simple Western lane view shows a specific band for PGRMC1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PGRMC1 Antibody [NBP1-83220] - Electropherogram image(s) of corresponding Simple Western lane view. PGRMC1 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGRMC1 Antibody [NBP1-83220] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGRMC1 Antibody [NBP1-83220] - Analysis in human cell line HEK 293.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGRMC1 Antibody [NBP1-83220] - Analysis in control (vector only transfected HEK293T lysate) and PGRMC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGRMC1 Antibody [NBP1-83220] - Analysis in human liver tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular and stromal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGRMC1 Antibody [NBP1-83220] - Staining in human liver and skeletal muscle tissues . Corresponding PGRMC1 RNA-seq data are presented for the same tissues.
