Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-38524 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MID1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Midline-1/MID1 in extracts from 293 cells using a Midline-1/MID1 polyclonal antibody (Product # PA5-38524).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MID1 Polyclonal Antibody (Product # PA5-38524) and 75 kDa band corresponding to MID1 was observed across cell lines and tissue extract tested and was also enhanced upon Tumor Necrosis Factor Alpha and Thapsigargin treatment in A549 cells. Whole cell extracts (30 µg lysate) of A549 (Lane1), A549 treated with TNF alpha (200 ng/mL for 72h) (Lane2), A549 treated with Thapsigargin (0.8 umol/L) (Lane 3), HeLa (Lane 4), HEK-293 (Lane 5), K-562 (Lane 6) and tissue extract of Mouse Testis (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0332BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Midline-1/MID1 in HeLa cells using a Midline-1/MID1 polyclonal antibody (Product # PA5-38524).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Midline-1/MID1 in HeLa cells using a Midline-1/MID1 polyclonal antibody (Product # PA5-38524).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Midline-1/MID1 in paraffin-embedded human brain tissue using a Midline-1/MID1 polyclonal antibody (Product # PA5-38524).