Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- OAAB00689 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- BCKDHB antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- BCKDHB Antibody (N-term) western blot analysis in Hela cell lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).
- Sample type
- Hela cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the BCKDHB gene.
- Sample type
- lysatestransiently transfected with the BCKDHB gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- BCKDHB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human hepatocarcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol