ABIN950620

antibody from antibodies-online

Targeting: BCKDHB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN950620

Provider

antibodies-online

Product name

anti-Branched Chain Keto Acid Dehydrogenase E1, beta Polypeptide (BCKDHB) (N-Term), (AA 44-74) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 44-74 amino acids from the N-terminal region of human BCKDHB

Description

Affinity chromatography on Protein A

Reactivity

Human

Host

Rabbit

Epitope

N-Term,AA 44-74

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Branched chain alpha-keto acid dehydrogenase, E1-beta subunit gene is associated with premature ovarian failure.

Kang H, Lee SK, Cho SW, Lee SH, Kwack K
Fertility and sterility 2008 Mar;89(3):728-31

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Molecular and structural analyses of maple syrup urine disease and identification of a founder mutation in a Portuguese Gypsy community.

Quental S, Macedo-Ribeiro S, Matos R, Vilarinho L, Martins E, Teles EL, Rodrigues E, Diogo L, Garcia P, Eusébio F, Gaspar A, Sequeira S, Furtado F, Lança I, Amorim A, Prata MJ
Molecular genetics and metabolism 2008 Jun;94(2):148-56

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Description of the mutations in 15 subjects with variant forms of maple syrup urine disease.

Flaschker N, Feyen O, Fend S, Simon E, Schadewaldt P, Wendel U
Journal of inherited metabolic disease 2007 Nov;30(6):903-9

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Role of macrophage oxidative burst in the action of anthrax lethal toxin.

Hanna PC, Kruskal BA, Ezekowitz RA, Bloom BR, Collier RJ
Molecular medicine (Cambridge, Mass.) 1994 Nov;1(1):7-18

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term) (Cat. #AP50353PU-N). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BCKDHB Antibody (N-term) (Cat. #AP50353PU-N) western blot analysis in Hela cell line lysates (35μg/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of BCKDHB Antibody (N-term)(Cat#AP50353PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BCKDHB Antibody (N-term) (Cat. #AP50353PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BCKDHB Antibody (N-term) (Cat. #AP50353PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.