Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27725 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GABBR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG, SK-N-SH, C8D30.
- Concentration
- 0.43 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABBR1 Polyclonal Antibody detects GABBR1 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.7.5 % SDS-PAGE. GABBR1 Polyclonal Antibody (Product # PA5-27725) dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABBR1 Polyclonal Antibody detects GABBR1 protein by Western blot analysis. A. 30 µg PC-3 whole cell lysate/extract. B. 30 µg U87-MG whole cell lysate/extract. C. 30 µg SK-N-SH whole cell lysate/extract.7.5 % SDS-PAGE. GABBR1 Polyclonal Antibody (Product # PA5-27725) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GABBR1 was performed in SH-SY5Y cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABBR1 Polyclonal Antibody (Product # PA5-27725) diluted at 1:500. Red: SOX2 protein stained by SOX2 antibody. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GABBR1 was performed in U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABBR1 Polyclonal Antibody (Product # PA5-27725) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GABBR1 was performed in SH-SY5Y cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GABBR1 Polyclonal Antibody (Product # PA5-27725) diluted at 1:500. Red: SOX2 protein stained by SOX2 antibody. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GABBR1 was performed in paraffin-embedded rat brain tissue using GABBR1 Polyclonal Antibody (Product # PA5-27725) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GABBR1 Polyclonal Antibody detects GABA B Receptor 1 protein at cytosol on human brain by immunohistochemical analysis. Sample: Paraffin-embedded human brain. GABBR1 Polyclonal Antibody (Product # PA5-27725) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.