Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-80973 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80973, RRID:AB_11026859
- Product name
- Rabbit Polyclonal OCRL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human OCRL antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Recruitment of OCRL and Inpp5B to phagosomes by Rab5 and APPL1 depletes phosphoinositides and attenuates Akt signaling.
Bohdanowicz M, Balkin DM, De Camilli P, Grinstein S
Molecular biology of the cell 2012 Jan;23(1):176-87
Molecular biology of the cell 2012 Jan;23(1):176-87
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: OCRL Antibody [NBP1-80973] - Analysis in human cell line EFO-21.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: OCRL Antibody [NBP1-80973] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-OCRL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OCRL Antibody [NBP1-80973] - Staining of human kidney shows cytoplasmic positivity in cells of tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OCRL Antibody [NBP1-80973] - Staining of human Fallopian tube shows moderate to strong cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OCRL Antibody [NBP1-80973] - Staining of human liver shows weak positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OCRL Antibody [NBP1-80973] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic and endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OCRL Antibody [NBP1-80973] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.