PA5-109306

antibody from Invitrogen Antibodies

Targeting: ACTN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109306

Provider

Invitrogen Antibodies

Product name

alpha Actinin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A431, rat colon tissue lysates, mouse colon tissue lysates, MG-63, SiHa, human colon cancer tissue, human kidney tissue, mouse colon tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Actinin in Lane 1: A431, Lane 2: Rat colon tissue, Lane 3: Mouse colon tissue . Samples were incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Actinin in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Actinin in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Actinin in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha Actinin in paraffin-embedded mouse colon tissue. Samples were incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha Actinin in paraffin-embedded human kidney tissue. Samples were incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha Actinin in paraffin-embedded human colon cancer tissue. Samples were incubated with alpha Actinin polyclonal antibody (Product # PA5-109306), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of alpha Actinin in MG-63 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Samples were incubated with alpha Actinin polyclonal antibody (Product # PA5-109306) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.