TA507228

antibody from OriGene

Targeting: ANKRD49 FGIF, FLJ20189, GBIF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA507228

Provider

OriGene

Proper citation

OriGene Cat#TA507228, RRID:AB_2624148

Product name

ANKRD49 mouse monoclonal antibody, clone OTI3E4 (formerly 3E4)

Antibody type

Monoclonal

Description

ANKRD49 mouse monoclonal antibody, clone OTI3E4 (formerly 3E4)

Host

Mouse

Conjugate

Unconjugated

Epitope

ANKRD49

Isotype

IgG

Antibody clone number

OTI3E4

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD49 (RC204657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD49.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANKRD49 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ANKRD49 mouse monoclonal antibody (TA507228) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD49(RC204657).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-ANKRD49 mouse monoclonal antibody (TA507228).

Validation comment

IF