MAB2238

antibody from R&D Systems

Targeting: KIR2DL4 103AS, 15.212, CD158D

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2238

Provider

R&D Systems

Product name

Human KIR2DL4/CD158d Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Stains human KIR2DL4/CD158d transfected BaF/3 cells. It does not recognize transfectants that express KIR2DL1, 2DL2, 2DL3, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q99706


Isotype

IgG

Antibody clone number

181703

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Killer cell immunoglobulin-like receptor 2DL4 is expressed in and suppresses the cell growth of Langerhans cell histiocytosis.

Takei Y, Ueshima C, Kataoka TR, Hirata M, Sugimoto A, Rokutan-Kurata M, Moriyoshi K, Ono K, Murakami I, Iwamoto S, Haga H
Oncotarget 2017 Jun 6;8(23):36964-36972

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Activating KIR2DS4 Is Expressed by Uterine NK Cells and Contributes to Successful Pregnancy.

Kennedy PR, Chazara O, Gardner L, Ivarsson MA, Farrell LE, Xiong S, Hiby SE, Colucci F, Sharkey AM, Moffett A
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4292-4300

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Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-γ responses to soluble HLA-G preparations.

Le Page ME, Goodridge JP, John E, Christiansen FT, Witt CS
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):732-40

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TNFR-associated factor 6 and TGF-β-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor.

Rajagopalan S, Lee EC, DuPrie ML, Long EO
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):714-21

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Human cytomegalovirus infection elicits new decidual natural killer cell effector functions.

Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N
PLoS pathogens 2013;9(4):e1003257

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Direct binding of human NK cell natural cytotoxicity receptor NKp44 to the surfaces of mycobacteria and other bacteria.

Esin S, Batoni G, Counoupas C, Stringaro A, Brancatisano FL, Colone M, Maisetta G, Florio W, Arancia G, Campa M
Infection and immunity 2008 Apr;76(4):1719-27

Other assay

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Human KIR2DL4/CD158d Antibody Induces IFN-gamma Secretion in NK-92 Cells. Human KIR2DL4/CD158d Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Quantikine Human IFN-gamma ELISA Kit (Catalog # DIF50). The ED₅₀ for this effect is typically 0.5-2 μg/mL.