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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-22413 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal AMIGO Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 60-80kDa depending on maturity/glycosylation.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: AMIGO Antibody (S86-36) [NBP2-22413] - analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 . Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.
