PA5-90325

antibody from Invitrogen Antibodies

Targeting: ATP5F1C ATP5C, ATP5C1, ATP5CL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90325

Provider

Invitrogen Antibodies

Product name

ATP Synthase gamma Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ATLKDITRRL KSIKNIQKIT KSMKMVAAAK YARAERELKP ARIYGLGSLA LYEKADIKGP EDKKKHLLIG VSSDRGLCGA IHSSIAKQMK SEVATLTAAG KEVMLVGIGD KIRGILYRTH SDQFLVAFKE VGRKPPTFGD ASVIALELLN SGYEFDEGSI IFNKFRSVIS YKTEEKPIFS LNTVASADSM SIYDDIDADV LQNYQEYNLA NIIYYSLKES TTSEQSARMT AMDNASKNAS EMIDKLTLTF NRTRQAVITK ELIEIISGAA ALD; Positive Samples: LO2, U-87MG, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat liver; Cellular Location: Mitochondrion, Mitochondrion inner membrane, Peripheral membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.38 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ATP Synthase gamma in extracts of various cell lines using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ATP Synthase gamma was performed in C6 cells using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ATP Synthase gamma was performed in L929 cells using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ATP Synthase gamma was performed in U-2 OS cells using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ATP Synthase gamma in paraffin-embedded human placenta using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ATP Synthase gamma in paraffin-embedded rat ovary using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ATP Synthase gamma in paraffin-embedded human lung cancer using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ATP Synthase gamma in paraffin-embedded human placenta using ATP Synthase gamma Polyclonal Antibody (Product # PA5-90325) at a dilution of 1:200.