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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-70309 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMCES Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMCES was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 105 mouse transformed pre-B cells transduced with or without guide RNA targeting HMCES (gHMCES). Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probed with a polyclonal anti-HMCES antibody (Product # PA5-70309) at a dilution of 1:1,000 in 2% milk/TBST at 4 deg overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5,000 at room temperature for 1 hour. Chemiluminescent detection was performed. Data courtesy of Antibody Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMCES on mouse small intestine tissue lysate. The sample was probed with a HMCES polyclonal antibody (Product # PA5-70309) using a primary antibody dilution of 1.0 µg/mL.
