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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-24199 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase VA/VB Monoclonal Antibody (487908)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6-14 is observed.
- Antibody clone number
- 487908
- Concentration
- 0.5 mg/mL
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Carbonic Anhydrase VA/VB in immersion fixed paraffin-embedded sections of human liver. Samples were incubated in Carbonic Anhydrase VA/VB monoclonal antibody (Product # MA5-24199) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes.
