PA5-95889

antibody from Invitrogen Antibodies

Targeting: CYP27B1 CYP1, P450c1, PDDR, VDD1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95889

Provider

Invitrogen Antibodies

Product name

CYP27B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LGYREYHSAR RSLADIPGPS TPSFLAELFC KGGLSRLHEL QVQGAAHFGP VWLASFGTVR TVYVAAPALV EELLRQEGPR PERCSFSPWT EHRRCRQRAC GLLTAEGEEW QRLRSLLAPL LLRPQAAARY AGTLNNVVCD LVRRLRRQRG RGTGPPALVR DVAGEFYKFG L; Positive Samples: Rat kidney, Rat liver; Cellular Location: Mitochondrion membrane

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.08 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CYP27B1 in extracts of various cell lines using CYP27B1 Polyclonal Antibody (Product # PA5-95889) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.