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antibody from Invitrogen Antibodies
Targeting: MED12
ARC240, CAGH45, FGS1, HOPA, KIAA0192, Kto, OKS, OPA1, TNRC11, TRAP230
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [9]
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- Product number
- PA5-51852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MED12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LPTTMTGVMG LEPSSYKTSV YRQQQPAVPQ GQRLRQQLQA KIQSQGMLGQ SSVHQMTPSS SYGLQTSQGY TPYVSHVGLQ QHTGPAGTMV PPSYSSQPYQ STHPSTNPTL VDPTRHLQQR PSGYVHQQAP TYGHGLTS
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MED12 in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a MED12 Polyclonal Antibody (Product # PA5-51852). Remaining relative intensity is presented.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MED12 Polyclonal Antibody (Product # PA5-51852) and a 250 kDa band corresponding to MED12 was observed across the panel tested except HT-29 and Caco-2 which are reported to be negative. Nuclear enriched extracts (30 µg lysate) of MCF 10A (Lane 1), MDA-MB-231 (Lane 2), SK-BR-3 (Lane 3), A-375 (Lane 4), SK-O-V3 (Lane 5), HT-29 (Lane 6) and Caco-2 (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MED12 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a MED12 Polyclonal Antibody (Product # PA5-51852).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MED12 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MED12 Polyclonal Antibody (Product # PA5-51852) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:3000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleus localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human testis using MED12 Polyclonal Antibody (Product # PA5-51852).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human testis using MED12 Polyclonal Antibody (Product # PA5-51852).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human rectum using a MED12 Polyclonal Antibody (Product # PA5-51852) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human cerebral cortex, colon, spleen and testis using MED12 Polyclonal Antibody (Product # PA5-51852) (A) shows similar protein distribution across tissues to an independent MED12 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human placenta using MED12 Polyclonal Antibody (Product # PA5-51852) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human pancreas using MED12 Polyclonal Antibody (Product # PA5-51852) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human cerebral cortex using MED12 Polyclonal Antibody (Product # PA5-51852).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human colon using MED12 Polyclonal Antibody (Product # PA5-51852).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MED12 in human spleen using MED12 Polyclonal Antibody (Product # PA5-51852).
