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LearnNB100-2357
antibody from Novus Biologicals
Targeting: MED12
ARC240, CAGH45, FGS1, HOPA, KIAA0192, Kto, OKS, OPA1, TNRC11, TRAP230
Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2357 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2357, RRID:AB_2281985
- Product name
- Rabbit Polyclonal MED12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas.
Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-βRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker.
Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription.
Sox10 cooperates with the mediator subunit 12 during terminal differentiation of myelinating glia.
Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines.
Control of ground-state pluripotency by allelic regulation of Nanog.
Core promoter recognition complex changes accompany liver development.
A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells.
Mosaic expression of Med12 in female mice leads to exencephaly, spina bifida, and craniorachischisis.
Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling.
MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.
Role for Med12 in regulation of Nanog and Nanog target genes.
Cao T, Jiang Y, Wang Z, Zhang N, Al-Hendy A, Mamillapalli R, Kallen AN, Kodaman P, Taylor HS, Li D, Huang Y
Oncogene 2019 Jul;38(27):5356-5366
Oncogene 2019 Jul;38(27):5356-5366
Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-βRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker.
Yokouchi H, Nishihara H, Harada T, Ishida T, Yamazaki S, Kikuchi H, Oizumi S, Uramoto H, Tanaka F, Harada M, Akie K, Sugaya F, Fujita Y, Takamura K, Kojima T, Higuchi M, Honjo O, Minami Y, Watanabe N, Goto A, Suzuki H, Dosaka-Akita H, Isobe H, Nishimura M, Munakata M
Oncotarget 2017 Jun 13;8(24):39711-39726
Oncotarget 2017 Jun 13;8(24):39711-39726
Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription.
Wagner LM, DeLuca NA
PloS one 2013;8(10):e78242
PloS one 2013;8(10):e78242
Sox10 cooperates with the mediator subunit 12 during terminal differentiation of myelinating glia.
Vogl MR, Reiprich S, Küspert M, Kosian T, Schrewe H, Nave KA, Wegner M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Apr 10;33(15):6679-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Apr 10;33(15):6679-90
Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines.
Valdez BC, Nieto Y, Murray D, Li Y, Wang G, Champlin RE, Andersson BS
Experimental hematology 2012 Oct;40(10):800-10
Experimental hematology 2012 Oct;40(10):800-10
Control of ground-state pluripotency by allelic regulation of Nanog.
Miyanari Y, Torres-Padilla ME
Nature 2012 Feb 12;483(7390):470-3
Nature 2012 Feb 12;483(7390):470-3
Core promoter recognition complex changes accompany liver development.
D'Alessio JA, Ng R, Willenbring H, Tjian R
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar 8;108(10):3906-11
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar 8;108(10):3906-11
A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells.
Langlais D, Couture C, Sylvain-Drolet G, Drouin J
Molecular endocrinology (Baltimore, Md.) 2011 Feb;25(2):348-59
Molecular endocrinology (Baltimore, Md.) 2011 Feb;25(2):348-59
Mosaic expression of Med12 in female mice leads to exencephaly, spina bifida, and craniorachischisis.
Rocha PP, Bleiss W, Schrewe H
Birth defects research. Part A, Clinical and molecular teratology 2010 Aug;88(8):626-32
Birth defects research. Part A, Clinical and molecular teratology 2010 Aug;88(8):626-32
Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling.
Rocha PP, Scholze M, Bleiss W, Schrewe H
Development (Cambridge, England) 2010 Aug;137(16):2723-31
Development (Cambridge, England) 2010 Aug;137(16):2723-31
MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.
Ding N, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Boyer TG
The Journal of biological chemistry 2009 Jan 30;284(5):2648-56
The Journal of biological chemistry 2009 Jan 30;284(5):2648-56
Role for Med12 in regulation of Nanog and Nanog target genes.
Tutter AV, Kowalski MP, Baltus GA, Iourgenko V, Labow M, Li E, Kadam S
The Journal of biological chemistry 2009 Feb 6;284(6):3709-18
The Journal of biological chemistry 2009 Feb 6;284(6):3709-18
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED12 Antibody [NB100-2357] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-2357 used for WB at 0.1 mcg/ml (A) and at 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED12 Antibody [NB100-2357] - Detection of Human and Mouse Med12 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody NB100-2357 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MED12 Antibody [NB100-2357] - Detection of human Med12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Med12 antibody NB100-2357 (lot NB100-2357-2) used for IP at 6 ug per reaction. Med12 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2357-1). For blotting immunoprecipitated Med12, NB100-2357 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
