14-1239-82

IL3RA antibody from Invitrogen Antibodies
CD123
Immunohistochemistry
Recommended by provider
Flow cytometry
Supportive data in Antibodypedia

Antibody data

Product number
14-1239-82
Provider
Invitrogen Antibodies
Product name
Anti-CD123 Monoclonal Antibody (6H6), eBioscience™
Provider product page
Invitrogen Antibodies - 14-1239-82
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: The 6H6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
6H6
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
Provider Type Product Number
- No reagents -