Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX31141 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX31141, RRID:AB_10720926
- Product name
- vGluT3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse
- Host
- Rabbit
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot: VGLUT3 Antibody [GTX31141] - mouse brain lysate (10% gel, 1:29; reduced, denatured) using Rabbit antibody to c-terminal region of mouse VGluT3: whole serum at 1:500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBS (0.1 x), 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry: VGluT3 Antibody [GTX31141] - IHC on rat spinal cord (free floating sections) using Rabbit antibody to c-terminal region of VGluT3 (Vesicular glutamate transporter 3): whole serum at 1 : 300 dilution incubated overnight at room temperature and developed with DAB/Ni.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry: VGluT3 Antibody [GTX31141] - IHC on rat spinal cord (free floating sections) using Rabbit antibody to c-terminal region of VGluT3 (Vesicular glutamate transporter 3): whole serum at 1 : 300 dilution incubated overnight at room temperature and developed with DAB/Ni.