GTX15842

antibody from GeneTex

Targeting: PECAM1 CD31

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

GTX15842

Provider

GeneTex

Product name

CD31 antibody [HEC7]

Antibody type

Monoclonal

Reactivity

Human, Rat, Bovine

Host

Mouse

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of CD31 (green) in THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with CD31/PECAM-1 antibody [HEC7] in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4¢XC in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of CD31 in BAEC cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with CD31/PECAM-1 antibody [HEC7] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated in a proper secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FACS analysis of PBMC cells using CD31 antibody [HEC7] (green) or an isotype control (blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FACS analysis of PBMC cells using CD31 antibody [HEC7].