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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- UM500029 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500029, RRID:AB_2629043
- Product name
- PECAM1 mouse monoclonal antibody,clone UMAB29
- Antibody type
- Monoclonal
- Description
- PECAM1 mouse monoclonal antibody,clone UMAB29
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PECAM1
- Isotype
- IgG
- Antibody clone number
- UMAB29
- Vial size
- 100 µl
- Concentration
- 3.49mg/ml
Submitted references Visualization and quantification of mitochondrial structure in the endothelium of intact arteries.
Durand MJ, Ait-Aissa K, Levchenko V, Staruschenko A, Gutterman DD, Beyer AM
Cardiovascular research 2019 Aug 1;115(10):1546-1556
Cardiovascular research 2019 Aug 1;115(10):1546-1556
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECAM1 monoclonal antibody (Clone UMAB29) at 1:500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-PECAM1 monoclonal antibody (clone UMAB25, 1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB29, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB29, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB29, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PECAM1mouse monoclonal antibody. (Clone UMAB29, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human skin using anti-PECAM clone UMAB29 mouse monoclonal antibody (UM500029) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the endothelia cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using anti-PECAM clone UMAB29 mouse monoclonal antibody (UM500029) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the endothelia cells and weak staining in the trophoblast.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (Clone UMAB29). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 (Clone UMAB29) very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.
- Validation comment
- 10K-CHIP
