102-PA07

antibody from ReliaTech GmbH

Targeting: PECAM1 CD31

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

102-PA07

Provider

ReliaTech GmbH

Product name

Anti-human CD31/PECAM-1

Antibody type

Polyclonal

Antigen

Other

Description

antibody Protein-A purified from serum

Reactivity

Human

Host

Rabbit

Antigen sequence

QENSFTINSV DMKSLPDWTV QNGKNLTLQC FA
DVSTTSHV KPQHQMLFYK DDVLFYNISS MKST
ESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEY
QVLV EGVPSPRVTL DKKEAIQGGI VRVNCSVP
EE KAPIHFTIEK LELNEKMVKL KREKNSRDQN
FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ T
SESTKSELV TVTESFSTPK FHISPTGMIM EGA
QLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHN
RHGNK AVYSVMAMVE HSGNYTCKVE SSRISKV
SSI VVNITELFSK PELESSFTHL DQGERLNLS
C SIPGAPPANF TIQKEDTIVS QTQDFTKIAS
KSDSGTYICT AGIDKVVKKS NTVQIVVCEM LS
QPRISYDA QFEVIKGQTI EVRCESISGT LPIS
YQLLKT SKVLENSTKN SNDPAVFKDN PTEDVE
YQCV ADNCHSHAKM LSEVLRVKVI APVDEVQI
SI LSSKVVESGE DIVLQCAVNE GSGPITYKFY
REKEGKPFYQ MTSNATQAFW TKQKASKEQE G
EYYCTAFNR ANHASSVPRS KILTVRVILA PWK
KTRHHHH HH


Vial size

200 µg

Storage

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Western analysis of recombinant human sCD31 [Cat# S01-071] using a rabbit anti-human CD31 polyclonal antibody.

Sample type

Purified Protein

Immunocytochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Immunofluorescence staining of human CD31 in primary human dermal lymphatic endothelial cells (HDLEC) and primary human umbilical vein endothelial cells (HUVEC) with a polyclonal rabbit anti-human CD31 antibody [Cat# 102-PA07]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].

Sample type

HUVE cells

Immunohistochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human CD31 (5µg/ml) [Cat# 102-PA07] and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.

Sample type

cryo-sections of vasa vasorum of a human lymphatic collector