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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00005175-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005175-M01, RRID:AB_425586
- Product name
- PECAM1 monoclonal antibody (M01), clone 1D2-1A5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PECAM1.
- Antigen sequence
ENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVST
TSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPE
VRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSP
RVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKL
ELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLS
FRCQARIISGIHMQTSESTKSELVTVTESFSTPKF
HISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQ
KDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVES
SRISKVSSIVVNITELFSKPELESSFTHLDQGERL
NLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASK
SDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRI
SYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTS
KVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHS
HAKMLSEVLRVKVIAPVDEVQISILSSKVVESGED
IVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNAT
QAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSK
ILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCY
FLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNME
ANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEV
QVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRY
SRTEGSLDGT- Isotype
- IgG
- Antibody clone number
- 1D2-1A5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PECAM1 monoclonal antibody (M01), clone 1D2-1A5. Western Blot analysis of PECAM1 expression in human placenta.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PECAM1 expression in transfected 293T cell line by PECAM1 monoclonal antibody (M01), clone 1D2-1A5.Lane 1: PECAM1 transfected lysate(82.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PECAM1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PECAM1 transfected lysate using anti-PECAM1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PECAM1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PECAM1 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. [antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
