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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-23972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF20 Monoclonal Antibody (272317)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -18, -19, -23, recombinant mouse FGF-8b, -8c, -15, -23, rhFGF acidic, or rhFGF basic is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 272317
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry analysis of FGF20 in immersion fixed SW480 human colorectal adenocarcinoma cell line. Samples were incubated in FGF20 monoclonal antibody (Product # MA5-23972) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
