Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB120-20646-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Asparaginase Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination.
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Asparaginase Antibody [Biotin] [NB120-20646] - Lane 1: L-Asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin Conjugated L-Asparaginase antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 32 kDa for L-Asparaginase. Other band(s): L-Asparaginase splice variants and isoforms.