ALX-804-604-C100

antibody from Enzo Life Sciences

Targeting: CKAP4 CLIMP-63, ERGIC-63, P63

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ALX-804-604-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-604-C100, RRID:AB_2051140

Product name

CLIMP-63 monoclonal antibody (G1/296)

Antibody type

Monoclonal

Antigen

Purified from natural sources

Description

Protein G-affinity purified and gel filtrated (FPLC).

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

G1/296

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Determinants for membrane association of the hepatitis C virus NS2 protease domain.

Lange CM, Bellecave P, Dao Thi VL, Tran HT, Penin F, Moradpour D, Gouttenoire J
Journal of virology 2014 Jun;88(11):6519-23

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Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.

Chang J, Lee S, Blackstone C
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 10;110(37):14954-9

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Differential NDR/LATS interactions with the human MOB family reveal a negative role for human MOB2 in the regulation of human NDR kinases.

Kohler RS, Schmitz D, Cornils H, Hemmings BA, Hergovich A
Molecular and cellular biology 2010 Sep;30(18):4507-20

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Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46.

Breuza L, Halbeisen R, Jenö P, Otte S, Barlowe C, Hong W, Hauri HP
The Journal of biological chemistry 2004 Nov 5;279(45):47242-53

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Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment.

Klopfenstein DR, Klumperman J, Lustig A, Kammerer RA, Oorschot V, Hauri HP
The Journal of cell biology 2001 Jun 11;153(6):1287-300

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A novel direct interaction of endoplasmic reticulum with microtubules.

Klopfenstein DR, Kappeler F, Hauri HP
The EMBO journal 1998 Nov 2;17(21):6168-77

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The plasma cell associated antigen detectable by antibody VS38 is the p63 rough endoplasmic reticulum protein.

Banham AH, Turley H, Pulford K, Gatter K, Mason DY
Journal of clinical pathology 1997 Jun;50(6):485-9

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Reassessment of the subcellular localization of p63.

Schweizer A, Rohrer J, Slot JW, Geuze HJ, Kornfeld S
Journal of cell science 1995 Jun;108 ( Pt 6):2477-85

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Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers.

Schweizer A, Rohrer J, Hauri HP, Kornfeld S
The Journal of cell biology 1994 Jul;126(1):25-39

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A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein.

Schweizer A, Rohrer J, Jenö P, DeMaio A, Buchman TG, Hauri HP
Journal of cell science 1993 Mar;104 ( Pt 3):685-94

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Characterization of a novel 63 kDa membrane protein. Implications for the organization of the ER-to-Golgi pathway.

Schweizer A, Ericsson M, Bächi T, Griffiths G, Hauri HP
Journal of cell science 1993 Mar;104 ( Pt 3):671-83

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunocytochemistry using MAb to CLIMP-63 (G1/296) (Prod. No. ALX-804-604) at a 1:1'000 dilution.