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- Antigen structure
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- Western blot [1]
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- Product number
- MA1-195 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Nurr1 Monoclonal Antibody (N1404)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-195 detects Nurr1 in human samples, and does not recognize human NGF-B alpha and gamma. For reducing conditions, Western blot should be performed using an antibody dilution of 1 µg/mL and a dilution of 3 µg/mL for non-reducing conditions.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N1404
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Submitted references Transcriptional Profiling of Monocytes Deficient in Nuclear Orphan Receptors NR4A2 and NR4A3 Reveals Distinct Signalling Roles Related to Antigen Presentation and Viral Response.
Phelan DE, Shigemura M, Aldhafiri S, Mota C, Hall TJ, Sznajder JI, Murphy EP, Crean D, Cummins EP
Frontiers in immunology 2021;12:676644
Frontiers in immunology 2021;12:676644
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nurr1 was performed by loading 20 µg of HEK293 lysate overexpressing human Nurr1 (right lane B) or empty vector control (left lane A) and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. Recombinant Nurr1 was detected at ~70 kDa using a Nurr1 mouse monoclonal antibody (Product # MA1-195) at a dilution of 1 µg/mL in 5% Milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nurr1 in midbrain dopaminergic neurons derived from human pluripotent stem cells using Dopaminergic Neuron Differentiation Kit (Product # A147701). The cells were fixed with 2-step fixation with 4% paraformaldehyde in PBS for 15 minutes (Product # A29515), then permeabilized and blocked with 0.3% Triton X-100 /1% BSA in PBS (TB Buffer) for 30 minutes. Cells were double stained with a Nurr1 mouse monoclonal antibody (Red, Product # MA1-195) and a Tyrosine Hydroxylase rabbit polyclonal antibody (Green, Product # P21962) at a dilution of 1:1000 each in TB buffer overnight 4C. Cells were then labeled with both a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11029), and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A-11037) at a dilution of 1:1000 for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). Images were taken on Nikon T2000 at 20X magnification.
