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LearnMAB7578
antibody from Novus Biologicals
Targeting: NLRP3
AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MAB7578 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal NLRP3/NALP3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse NLRP3/NALP3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome.
Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats.
NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus.
Endothelial GPR124 Exaggerates the Pathogenesis of Atherosclerosis by Activating Inflammation.
Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation.
Evidence that TLR4 Is Not a Receptor for Saturated Fatty Acids but Mediates Lipid-Induced Inflammation by Reprogramming Macrophage Metabolism.
Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy.
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.
The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock.
Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice.
Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn.
NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma.
Intestinal fungi contribute to development of alcoholic liver disease.
Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light.
Suppression of NLRP3 inflammasome by γ-tocotrienol ameliorates type 2 diabetes.
Chinese Herbal Preparation Xuebijing Potently Inhibits Inflammasome Activation in Hepatocytes and Ameliorates Mouse Liver Ischemia-Reperfusion Injury.
The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
ASC in renal collecting duct epithelial cells contributes to inflammation and injury after unilateral ureteral obstruction.
Samir P, Kesavardhana S, Patmore DM, Gingras S, Malireddi RKS, Karki R, Guy CS, Briard B, Place DE, Bhattacharya A, Sharma BR, Nourse A, King SV, Pitre A, Burton AR, Pelletier S, Gilbertson RJ, Kanneganti TD
Nature 2019 Sep;573(7775):590-594
Nature 2019 Sep;573(7775):590-594
Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats.
Chang CY, Shih HJ, Huang IT, Tsai PS, Chen KY, Huang CJ
International journal of molecular sciences 2019 Sep 18;20(18)
International journal of molecular sciences 2019 Sep 18;20(18)
NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus.
Ferreira NS, Bruder-Nascimento T, Pereira CA, Zanotto CZ, Prado DS, Silva JF, Rassi DM, Foss-Freitas MC, Alves-Filho JC, Carlos D, Tostes RC
Cells 2019 Dec 8;8(12)
Cells 2019 Dec 8;8(12)
Endothelial GPR124 Exaggerates the Pathogenesis of Atherosclerosis by Activating Inflammation.
Gong DM, Zhang YL, Chen DY, Hong LJ, Han F, Liu QB, Jiang JJ, Lu YM
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(2):547-557
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(2):547-557
Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation.
Gugliandolo A, Giacoppo S, Ficicchia M, Aliquò A, Bramanti P, Mazzon E
Molecular medicine reports 2018 May;17(5):6235-6244
Molecular medicine reports 2018 May;17(5):6235-6244
Evidence that TLR4 Is Not a Receptor for Saturated Fatty Acids but Mediates Lipid-Induced Inflammation by Reprogramming Macrophage Metabolism.
Lancaster GI, Langley KG, Berglund NA, Kammoun HL, Reibe S, Estevez E, Weir J, Mellett NA, Pernes G, Conway JRW, Lee MKS, Timpson P, Murphy AJ, Masters SL, Gerondakis S, Bartonicek N, Kaczorowski DC, Dinger ME, Meikle PJ, Bond PJ, Febbraio MA
Cell metabolism 2018 May 1;27(5):1096-1110.e5
Cell metabolism 2018 May 1;27(5):1096-1110.e5
Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy.
Boursereau R, Abou-Samra M, Lecompte S, Noel L, Brichard SM
BMC biology 2018 Mar 20;16(1):33
BMC biology 2018 Mar 20;16(1):33
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.
Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG
Scientific reports 2018 Jan 11;8(1):461
Scientific reports 2018 Jan 11;8(1):461
The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock.
Chen X, Wang N, Zhu Y, Lu Y, Liu X, Zheng J
Mediators of inflammation 2017;2017:6543237
Mediators of inflammation 2017;2017:6543237
Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice.
DeWolf SE, Shigeoka AA, Scheinok A, Kasimsetty SG, Welch AK, McKay DB
Nephron 2017;137(1):68-76
Nephron 2017;137(1):68-76
Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn.
Bian F, Xiao Y, Zaheer M, Volpe EA, Pflugfelder SC, Li DQ, de Paiva CS
International journal of molecular sciences 2017 Mar 5;18(3)
International journal of molecular sciences 2017 Mar 5;18(3)
NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma.
Daley D, Mani VR, Mohan N, Akkad N, Pandian GSDB, Savadkar S, Lee KB, Torres-Hernandez A, Aykut B, Diskin B, Wang W, Farooq MS, Mahmud AI, Werba G, Morales EJ, Lall S, Wadowski BJ, Rubin AG, Berman ME, Narayanan R, Hundeyin M, Miller G
The Journal of experimental medicine 2017 Jun 5;214(6):1711-1724
The Journal of experimental medicine 2017 Jun 5;214(6):1711-1724
Intestinal fungi contribute to development of alcoholic liver disease.
Yang AM, Inamine T, Hochrath K, Chen P, Wang L, Llorente C, Bluemel S, Hartmann P, Xu J, Koyama Y, Kisseleva T, Torralba MG, Moncera K, Beeri K, Chen CS, Freese K, Hellerbrand C, Lee SM, Hoffman HM, Mehal WZ, Garcia-Tsao G, Mutlu EA, Keshavarzian A, Brown GD, Ho SB, Bataller R, Stärkel P, Fouts DE, Schnabl B
The Journal of clinical investigation 2017 Jun 30;127(7):2829-2841
The Journal of clinical investigation 2017 Jun 30;127(7):2829-2841
Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light.
Hu Z, Zhang Y, Wang J, Mao P, Lv X, Yuan S, Huang Z, Ding Y, Xie P, Liu Q
Cell death & disease 2016 Nov 10;7(11):e2468
Cell death & disease 2016 Nov 10;7(11):e2468
Suppression of NLRP3 inflammasome by γ-tocotrienol ameliorates type 2 diabetes.
Kim Y, Wang W, Okla M, Kang I, Moreau R, Chung S
Journal of lipid research 2016 Jan;57(1):66-76
Journal of lipid research 2016 Jan;57(1):66-76
Chinese Herbal Preparation Xuebijing Potently Inhibits Inflammasome Activation in Hepatocytes and Ameliorates Mouse Liver Ischemia-Reperfusion Injury.
Liu X, Hu Z, Zhou B, Li X, Tao R
PloS one 2015;10(7):e0131436
PloS one 2015;10(7):e0131436
The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
Maier NK, Leppla SH, Moayeri M
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2776-85
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 15;194(6):2776-85
Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
Antonopoulos C, Russo HM, El Sanadi C, Martin BN, Li X, Kaiser WJ, Mocarski ES, Dubyak GR
The Journal of biological chemistry 2015 Aug 14;290(33):20167-84
The Journal of biological chemistry 2015 Aug 14;290(33):20167-84
ASC in renal collecting duct epithelial cells contributes to inflammation and injury after unilateral ureteral obstruction.
Komada T, Usui F, Shirasuna K, Kawashima A, Kimura H, Karasawa T, Nishimura S, Sagara J, Noda T, Taniguchi S, Muto S, Nagata D, Kusano E, Takahashi M
The American journal of pathology 2014 May;184(5):1287-98
The American journal of pathology 2014 May;184(5):1287-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse NLRP3/NALP3 by Western Blot. Detection of Mouse NLRP3/NALP3 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- NLRP3/NALP3 in Human Tonsil Tissue. NLRP3/NALP3 in Human Tonsil Tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of NLRP3/NALP3 in Human Monocytes by Flow Cytometry. Detection of NLRP3/NALP3 in Human Monocytes by Flow Cytometry.
