PA5-20838
antibody from Invitrogen Antibodies
Targeting: NLRP3
AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Other assay [2]
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Validation data
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- Product number
- PA5-20838 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLRP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is K562 cell lysate. PA5-20838 can be used with blocking peptide PEP-0952.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Modafinil Reduces Neuronal Pyroptosis and Cognitive Decline After Sleep Deprivation.
Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice.
Role of Surfactant Protein D in Experimental Otitis Media.
TRIM31 inhibits NLRP3 inflammasome and pyroptosis of retinal pigment epithelial cells through ubiquitination of NLRP3.
Surfactant Protein D Dampens Lung Injury by Suppressing NLRP3 Inflammasome Activation and NF-κB Signaling in Acute Pancreatitis.
Xiong X, Zuo Y, Cheng L, Yin Z, Hu T, Guo M, Han Z, Ge X, Li W, Wang Y, Wang D, Wang C, Zhang L, Zhang Y, Liu Q, Chen F, Lei P
Frontiers in neuroscience 2022;16:816752
Frontiers in neuroscience 2022;16:816752
Malaria parasite heme biosynthesis promotes and griseofulvin protects against cerebral malaria in mice.
Chandana M, Anand A, Ghosh S, Das R, Beura S, Jena S, Suryawanshi AR, Padmanaban G, Nagaraj VA
Nature communications 2022 Jul 12;13(1):4028
Nature communications 2022 Jul 12;13(1):4028
Role of Surfactant Protein D in Experimental Otitis Media.
Abdel-Razek O, Liu T, Chen X, Wang Q, Vanga G, Wang G
Journal of innate immunity 2021;13(4):197-210
Journal of innate immunity 2021;13(4):197-210
TRIM31 inhibits NLRP3 inflammasome and pyroptosis of retinal pigment epithelial cells through ubiquitination of NLRP3.
Huang P, Liu W, Chen J, Hu Y, Wang Y, Sun J, Feng J
Cell biology international 2020 Nov;44(11):2213-2219
Cell biology international 2020 Nov;44(11):2213-2219
Surfactant Protein D Dampens Lung Injury by Suppressing NLRP3 Inflammasome Activation and NF-κB Signaling in Acute Pancreatitis.
Yu J, Ni L, Zhang X, Zhang J, Abdel-Razek O, Wang G
Shock (Augusta, Ga.) 2019 May;51(5):557-568
Shock (Augusta, Ga.) 2019 May;51(5):557-568
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of K562 cell lysate using a NALP3 polyclonal antibody (Product # PA5-20838) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NALP3 in K562 cell lysate with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 1 µg/mL (A) in the absence and (B) in the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of K562 cells using a NALP3 polyclonal antibody (Product # PA5-20838) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of NALP3 in K562 cells with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NALP3 in K562 cells with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NALP3 in K562 cells with NLRP3 Polyclonal Antibody (Product # PA5-20838) at 5 µg/mL. Red: NALP3 Blue: DAPI staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 Figure Effect of TRIM31 overexpression on NLRP3 ubiquitination. (a) Confirmation of the interactions between TRIM31 and NLRP3 by coimmunoprecipitation and western blot analysis in human RPE cells. (b) ARPE-19 cells were transfected with vector or plasmid overexpressing TRIM31 (oeTRIM31) for 48 hr and subjected to immunoprecipitation experiments. Anti-ubiquitin (Ub) detected NLRP3 ubiquitination in each group. IgG, immunoglobulin G; RPE, retinal pigment epithelial
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Pyroptosis induced by SD was inhibited by modafinil. (A,B) Western blot data showing that modafinil suppressed the increase in the expression of NLRP3, NLRC4, NLRP1, ASC, caspase-1, GSDMD, and the downstream pro-inflammatory cytokines IL-1beta and IL-18 in mice subjected to SD. All data presented are means +- SD; N = 4-5 mice per group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.