ALX-804-818-C100

antibody from Enzo Life Sciences

Targeting: NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCU, MWS, NALP3, PYPAF1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ALX-804-818-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-818-C100, RRID:AB_2051971

Product name

NLRP3/NALP3 (human) monoclonal antibody (Nalpy3-a)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

Nalpy3-a

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Reconstituted AIM2 inflammasome in cell-free system.

Kaneko N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J
Journal of immunological methods 2015 Nov;426:76-81

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NLRP3 Deficiency Improves Angiotensin II-Induced Hypertension But Not Fetal Growth Restriction During Pregnancy.

Shirasuna K, Karasawa T, Usui F, Kobayashi M, Komada T, Kimura H, Kawashima A, Ohkuchi A, Taniguchi S, Takahashi M
Endocrinology 2015 Nov;156(11):4281-92

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Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases.

Shio MT, Eisenbarth SC, Savaria M, Vinet AF, Bellemare MJ, Harder KW, Sutterwala FS, Bohle DS, Descoteaux A, Flavell RA, Olivier M
PLoS pathogens 2009 Aug;5(8):e1000559

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NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder.

Agostini L, Martinon F, Burns K, McDermott MF, Hawkins PN, Tschopp J
Immunity 2004 Mar;20(3):319-25

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunofluorescence microscopy showing the detection of endogenous NLRP3/NALP3 in THP1 cellsÊusing NLRP3/NALP3 (human), mAb (Nalpy3-a) (Prod. No. ALX-804-818). Method: Following fixation with paraformaldehyde 3.7%, THP1 cells were incubated with NLRP3/NALP3 (human), mAb (Nalpy3-a) (Prod. No. ALX-804-818) at a 1:100 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection.