MA5-29239

antibody from Invitrogen Antibodies

Targeting: ENO3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29239

Provider

Invitrogen Antibodies

Product name

ENO3 Recombinant Rabbit Monoclonal Antibody (3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

3

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ENO3 in Lane A: HepG2 Whole Cell Lysate (30 µg), Lane B: Raji Whole Cell Lysate (30 µg), Lane C: Hela Whole Cell Lysate (30 µg), Lane D: MOLT-4 Whole Cell Lysate (30 µg). Samples were probed using a ENO3 Monoclonal Antibody (Product # MA5-29239) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:47 kDa. Observed band size:50 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ENO3 Recombinant Rabbit Monoclonal Antibody (3) (Product # MA5-29239) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate, Lane C: Hela Whole Cell Lysate, Lane D: MOLT-4 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ENO3 in human skeletal muscle with ENO3 Recombinant Rabbit Monoclonal Antibody (3) (Product # MA5-29239, 1:200, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ENO3 Immunoprecipitation using: Lane A: 0.5 mg Raji Whole Cell Lysate 2 µL with ENO3 Recombinant Rabbit Monoclonal Antibody (3) (Product # MA5-29239) and 15 µL of 50 % Protein G agarose. Primary antibody: ENO3 Recombinant Rabbit Monoclonal Antibody (3), at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 53 kDa.