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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00026508-M07 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00026508-M07, RRID:AB_606375
- Product name
- HEYL monoclonal antibody (M07), clone 1F6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant HEYL.
- Antigen sequence
PLRRATGIILPARRNVLPSRGASSTRRARPLERPA
TPVPVAPSSRAARSSHIAPLLQSSSPTPPGPTGSA
AYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEI
GA- Isotype
- IgG
- Antibody clone number
- 1F6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Repression of androgen receptor activity by HEYL, a third member of the Hairy/Enhancer-of-split-related family of Notch effectors.
Lavery DN, Villaronga MA, Walker MM, Patel A, Belandia B, Bevan CL
The Journal of biological chemistry 2011 May 20;286(20):17796-808
The Journal of biological chemistry 2011 May 20;286(20):17796-808
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEYL monoclonal antibody (M07), clone 1F6 Western Blot analysis of HEYL expression in HeLa ( Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HEYL is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to HEYL on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HEYL on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
