NBP2-29627

antibody from Novus Biologicals

Targeting: GLI3 ACLS, GCPS, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-29627

Provider

Novus Biologicals

Product name

Rabbit Polyclonal GLI-3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Modulation of the IL-6 Receptor α Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenström Macroglobulinemia Cells.

Jackson DA, Smith TD, Amarsaikhan N, Han W, Neil MS, Boi SK, Vrabel AM, Tolosa EJ, Almada LL, Fernandez-Zapico ME, Elsawa SF
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2908-16

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GLI-3 Antibody [NBP2-29627] - Analysis of GLI-3 in Hela cell lysate and MEF cell lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GLI-3 Antibody [NBP2-29627] - Adult mouse brain tissue lysates. Image from verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse liver.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): GLI-3 Antibody [NBP2-29627] - Intracellular staining for GLI-3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-3 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.