AP09241PU-N

antibody from Acris Antibodies GmbH

Targeting: ATF3

ELISA

Antibody data

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Validation data

ATF3 protein structure - AP09241PU-N shown in red.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4Â°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's OdysseyÂ® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Anti-GST epitope tag antibodyconfirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. Afterblocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4Â°C. Reaction was followed bywashes and reaction with a 1:5000 dilution of IRDye(TM)800 conjugated Gta-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's OdysseyÂ® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.