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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-30378 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116.
- Concentration
- 1.63 mg/mL
Submitted references Modulation of epidermal transcription circuits in psoriasis: new links between inflammation and hyperproliferation.
Swindell WR, Johnston A, Xing X, Voorhees JJ, Elder JT, Gudjonsson JE
PloS one 2013;8(11):e79253
PloS one 2013;8(11):e79253
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRF9 using 30 µg of A) A549 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IRF9 polyclonal antibody (Product # PA5-30378) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IRF9 Polyclonal Antibody (Product # PA5-30378). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF9 Polyclonal Antibody (Product # PA5-30378) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRF9 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IRF9 Polyclonal Antibody (Product # PA5-30378) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IRF9 in paraformaldehyde-fixed HeLa cells using an IRF9 polyclonal antibody (Product # PA5-30378) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using IRF9 (Product # PA5-30378) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 STAT1 and IRF9 mRNA are elevated in psoriasis lesions with increased abundance of pSTAT1(ser727) and IRF9 protein in the psoriatic epidermis. Figures (A), (D) and (G) show the PP/PN fold-change distributions for STAT1 , IRF1 and IRF9 , respectively ( n = 215 patients). Figures (B), (E) and (H) show staining for pSTAT1(ser727), IRF1 and IRF9 in lesional (PP) skin, respectively. Figures (C), (F) and (I) show staining for pSTAT1(ser727), IRF1 and IRF9 in uninvolved (PN) skin, respectively.
