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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00001149-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001149-M01, RRID:AB_608453
- Product name
- Mouse Monoclonal CIDEA Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CIDEA - cell death-inducing DFFA-like effector a
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human adipose beiging in response to cold and mirabegron.
Immunotherapeutic potential of anti-human endogenous retrovirus-K envelope protein antibodies in targeting breast tumors.
Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
Differential regulation of CIDEA and CIDEC expression by insulin via Akt1/2- and JNK2-dependent pathways in human adipocytes.
Differential roles of CIDEA and CIDEC in insulin-induced anti-apoptosis and lipid droplet formation in human adipocytes.
Neuronal protein tyrosine phosphatase 1B deficiency results in inhibition of hypothalamic AMPK and isoform-specific activation of AMPK in peripheral tissues.
Cidea is associated with lipid droplets and insulin sensitivity in humans.
Finlin BS, Memetimin H, Confides AL, Kasza I, Zhu B, Vekaria HJ, Harfmann B, Jones KA, Johnson ZR, Westgate PM, Alexander CM, Sullivan PG, Dupont-Versteegden EE, Kern PA
JCI insight 2018 Aug 9;3(15)
JCI insight 2018 Aug 9;3(15)
Immunotherapeutic potential of anti-human endogenous retrovirus-K envelope protein antibodies in targeting breast tumors.
Wang-Johanning F, Rycaj K, Plummer JB, Li M, Yin B, Frerich K, Garza JG, Shen J, Lin K, Yan P, Glynn SA, Dorsey TH, Hunt KK, Ambs S, Johanning GL
Journal of the National Cancer Institute 2012 Feb 8;104(3):189-210
Journal of the National Cancer Institute 2012 Feb 8;104(3):189-210
Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
Pulinilkunnil T, He H, Kong D, Asakura K, Peroni OD, Lee A, Kahn BB
The Journal of biological chemistry 2011 Mar 18;286(11):8798-809
The Journal of biological chemistry 2011 Mar 18;286(11):8798-809
Differential regulation of CIDEA and CIDEC expression by insulin via Akt1/2- and JNK2-dependent pathways in human adipocytes.
Ito M, Nagasawa M, Omae N, Ide T, Akasaka Y, Murakami K
Journal of lipid research 2011 Aug;52(8):1450-60
Journal of lipid research 2011 Aug;52(8):1450-60
Differential roles of CIDEA and CIDEC in insulin-induced anti-apoptosis and lipid droplet formation in human adipocytes.
Ito M, Nagasawa M, Hara T, Ide T, Murakami K
Journal of lipid research 2010 Jul;51(7):1676-84
Journal of lipid research 2010 Jul;51(7):1676-84
Neuronal protein tyrosine phosphatase 1B deficiency results in inhibition of hypothalamic AMPK and isoform-specific activation of AMPK in peripheral tissues.
Xue B, Pulinilkunnil T, Murano I, Bence KK, He H, Minokoshi Y, Asakura K, Lee A, Haj F, Furukawa N, Catalano KJ, Delibegovic M, Balschi JA, Cinti S, Neel BG, Kahn BB
Molecular and cellular biology 2009 Aug;29(16):4563-73
Molecular and cellular biology 2009 Aug;29(16):4563-73
Cidea is associated with lipid droplets and insulin sensitivity in humans.
Puri V, Ranjit S, Konda S, Nicoloro SM, Straubhaar J, Chawla A, Chouinard M, Lin C, Burkart A, Corvera S, Perugini RA, Czech MP
Proceedings of the National Academy of Sciences of the United States of America 2008 Jun 3;105(22):7833-8
Proceedings of the National Academy of Sciences of the United States of America 2008 Jun 3;105(22):7833-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CIDEA Antibody (4B9) [H00001149-M01] - Analysis of CIDEA expression in transfected 293T cell line by CIDEA monoclonal antibody (M01), clone 4B9.Lane 1: CIDEA transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CIDEA Antibody (4B9) [H00001149-M01] - Detection limit for recombinant GST tagged CIDEA is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CIDEA Antibody (4B9) [H00001149-M01] - Analysis of CIDEA transfected lysate using anti-CIDEA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CIDEA MaxPab rabbit polyclonal antibody.
