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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- GTX115186 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115186, RRID:AB_10620301
- Product name
- USP14 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteasomal activity-based probes mark protein homeostasis in muscles.
Raz V, Raz Y, Paniagua-Soriano G, Roorda JC, Olie C, Riaz M, Florea BI
Journal of cachexia, sarcopenia and muscle 2017 Oct;8(5):798-807
Journal of cachexia, sarcopenia and muscle 2017 Oct;8(5):798-807
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- USP14 antibody detects USP14 protein by Western blot analysis.A. 50 ?g mouse brain lysate/extract7.5 % SDS-PAGEUSP14 antibody (GTX115186) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: JurKat 7.5% SDS PAGE GTX115186 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- USP14 antibody detects USP14 protein by Western blot analysis.A. 50 ?g rat brain lysate/extract7.5 % SDS-PAGEUSP14 antibody (GTX115186) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115186) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using USP14(GTX115186) antibody at 1:500 dilution.
