PAB29362

antibody from Abnova Corporation

Targeting: USP14 TGT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29362

Provider

Abnova Corporation

Product name

USP14 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human USP14.

Antigen sequence

EKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVS
FRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPF
SFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSW
VKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHI
AYVL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with USP14 polyclonal antibody (Cat # PAB29362) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with USP14 polyclonal antibody (Cat # PAB29362) at 1-4 ug/mL concentration shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with USP14 polyclonal antibody (Cat # PAB29362) shows strong cytoplasmic positivity in exocrine glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)