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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00009097-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009097-M04, RRID:AB_549059
- Product name
- Mouse Monoclonal USP14 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. USP14 - ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Ubiquitin-specific protease-14 reduces cellular aggregates and protects against mutant huntingtin-induced cell degeneration: involvement of the proteasome and ER stress-activated kinase IRE1α.
Proteasome function is required for DNA damage response and fanconi anemia pathway activation.
Hyrskyluoto A, Bruelle C, Lundh SH, Do HT, Kivinen J, Rappou E, Reijonen S, Waltimo T, Petersén Å, Lindholm D, Korhonen L
Human molecular genetics 2014 Nov 15;23(22):5928-39
Human molecular genetics 2014 Nov 15;23(22):5928-39
Proteasome function is required for DNA damage response and fanconi anemia pathway activation.
Jacquemont C, Taniguchi T
Cancer research 2007 Aug 1;67(15):7395-405
Cancer research 2007 Aug 1;67(15):7395-405
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody (M04), clone 6D6. Lane 1: USP14 transfected lysatE (56.1 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in PC-12 (Cat # L012V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: USP14 Antibody (6D6) [H00009097-M04] - Detection limit for recombinant GST tagged USP14 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: USP14 Antibody (6D6) [H00009097-M04] - Analysis of monoclonal antibody to USP14 on HeLa cell. Antibody concentration 20 ug/ml
