PAB28411

antibody from Abnova Corporation

Targeting: SUB1 p14, p15, PC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28411

Provider

Abnova Corporation

Product name

SUB1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SUB1.

Antigen sequence

VSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKT
GETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDF
KGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQ
LKEQI


Isotype

IgG

Storage

Store at 4 °C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: A-431, lane 4: Liver and lane 5: Tonsil using SUB1 polyclonal antibody (Cat # PAB28411).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: NIH-3T3, lane 2: NBT-II and lane 3: PC12 cell lysates using SUB1 polyclonal antibody (Cat # PAB28411).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of U-2 OS cell line with SUB1 polyclonal antibody (Cat # PAB28411) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-100

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast with SUB1 polyclonal antibody (Cat # PAB28411) shows strong nuclear positivity in glandular cells.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)