R32566

antibody from NSJ Bioreagents

Targeting: SUB1 p14, p15, PC4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32566

Provider

NSJ Bioreagents

Product name

PC4 Antibody / Positive cofactor 4

Antibody type

Polyclonal (Antigen purified)

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the PC4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat liver, 2) human HeLa and 3) human U-2 OS lysate with PC4 antibody at 0.5ug/ml. Expected molecular weight: 15-19 kDa (unmodified) and ~26 kDa (phosphorylated).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with PC4 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse liver with PC4 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat liver with PC4 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.