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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-59775 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59775, RRID:AB_892400
- Product name
- Rabbit Polyclonal SUB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma.
Lin SJ, Chang KP, Hsu CW, Chi LM, Chien KY, Liang Y, Tsai MH, Lin YT, Yu JS
Journal of proteomics 2013 Dec 6;94:186-201
Journal of proteomics 2013 Dec 6;94:186-201
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SUB1 Antibody [NB100-59775] - Detection of human PC4. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-PC4 antibody NB100-59775 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SUB1 Antibody [NB100-59775] - Detection of human SUB1/PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUB1 antibody NB100-59775 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). SUB1 was immunoprecipitated by rabbit anti-SUB1 antibody NB100-59774, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PC4 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB
